TABLE 2.

ORFs whose expression is down-regulated within 10 min after the addition of elemental sulfur to growing P. furiosus cells

Function and ORFa	Description	Fold changeb
PF0450	[Glutamine synthetase, catalytic region]	4.2
[Cobalt transport]
PF0529d	[Cobalt transport protein (CbiQ)]	5.1
PF0530d	[Conserved hypothetical protein]	2.6
PF0531d	[Cobalamin (vitamin B12) biosynthesis CbiM]	5.6
PF0559	[Hydrogenase maturation protein HypF]	6.3
PF0736e	[Conserved hypothetical protein]	5.0
PF0736.1e	[Conserved hypothetical protein]	4.1
Hydrogenase I (31)
PF0891	Hydrogenase I beta	4.4
PF0892	Hydrogenase I gamma	4.9
PF0893	Hydrogenase I delta	4.0
PF0894	Hydrogenase I alpha	2.9
PF0913c	[Formylmethanofuran dehydrogenase, subunit E]	6.1
PF0915	[Cytochrome c biogenesis protein]	3.0
[Unknown]
PF0925f	[Radical SAM]	9.4
PF0926f	[Conserved hypothetical protein]	10.2
Hydrogenase IIg (32)
PF1329	Hydrogenase II beta	11.3
PF1330	Hydrogenase II gamma	7.8
PF1331	Hydrogenase II delta	10.0
PF1332	Hydrogenase II alpha	4.4
Membrane-bound hydrogenase (42)
PF1423	MbhA	4.4
PF1424	MbhB	7.2
PF1425	MbhC	6.5
PF1426	MbhD	8.0
PF1427	MbhE	6.8
PF1428	MbhF	6.7
PF1429	MbhG	ND
PF1430	MbhH	5.2
PF1431	MbhI	5.7
PF1432	MbhJ	ND
PF1433	MbhK	4.0
PF1434	MbhL	3.7
PF1435	MbhM	2.5
PF1436	MbhN	2.4
PF1621d	[Fibronectin, type III-like fold]	4.2

^aFor explanation, see Table 1, footnote a.

^bAll changes (n-fold) are statistically significant (P < 0.05) unless indicated. ND, not determined.

^cNo homolog present in the genome sequence of T. kodakaraensis.

^dNo homologs in the genome sequences of P. horikoshii and P. abyssi.

^eUnique to P. furiosus.

^fNo homologs in the genome sequences of T. kodakaraensis, P. horikoshii, and P. abyssi.

^gNo homologs in the genome sequences of T. kodakaraensis and P. horikoshii.