TABLE 3.

ORFs whose expression is up-regulated within 30 min after the addition of elemental sulfur to growing P. furiosus cells

Function and ORFa	Description	Fold changeb
[Glutamate biosynthesis]
PF0204f	[Glutamate synthase, large subunit]	4.0
PF0205f	[Ferredoxin-dependent glutamate synthase]	2.8
PF0206f	[Glutamate synthase, large subunit region 3]	3.2
PF0686e	[Hypothetical protein]	3.1
PF0704	[Protein of unknown function DUF302]	3.1
PF0857	[Ferrous iron transport protein B, N terminal]	3.2
[Branched-chain amino acid biosynthesis]
PF0935	[Acetolactate synthase, large subunit]	4.6
PF0936	[Acetohydroxy acid isomeroreductase]	4.7
PF0937	[Pyruvate carboxyltransferase]	5.0
PF0938	[3-Isopropylmalate dehydratase large subunit]	3.5
PF0939	[3-Isopropylmalate dehydratase small subunit]	4.3
PF0940	[Isocitrate/isopropylmalate dehydrogenase]	3.7
PF0941	[2-Isopropylmalate/homocitrate synthase]	3.1
PF0942	[6-Phosphogluconate dehydratase]	3.2
[Transcription factor]
PF0984	[Hypothetical protein]	3.3
PF0986	[Transcription factor TFIIS]	3.4
[Iron-sulfur cluster assembly]
PF1285f	[SufBD]	2.3
PF1286f	[SufBD]	3.2
[Branched-chain amino acid biosynthesis]
PF1678	[Alpha-isopropylmalate/homocitrate synthase]	5.2
PF1679	[3-Isopropylmalate dehydratase large subunit]	6.9
PF1680	[3-Isopropylmalate dehydratase small subunit]	4.9
[Ribosome]
PF1823	[Ribosomal L23 protein]	2.6
PF1824	[Ribosomal protein L4/L1e]	4.0
PF1852	[Glutamate synthase (21)]	3.4
[Sulfur-induced proteins(46)]
PF2025	SipA	9.3
PF2026	SipB	6.5
PF2029	[Hypothetical protein]	3.7

^aFor explanation, see Table 1, footnote a.

^bAll changes (n-fold) are statistically significant (P < 0.05) unless indicated. ND, not determined.

c No homolog present in the genome sequence of T. kodakaraensis.

d No homologs in the genome sequences of P. horikoshii and P. abyssi.

^eUnique to P. furiosus.

^fNo homologs in the genome sequences of T. kodakaraensis, P. horikoshii, and P. abyssi.