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TcpR and TcpT interaction is demonstrated by metal affinity chromatography. Whole-cell extracts from a tcpR deletion strain and tcpR complemented with pTcpR-6xHis grown under tcpR::6xhis-inducing (arabinose +) and noninducing (arabinose −) conditions were solubilized with 1% Triton X-100 and used for metal affinity chromatography as discussed in Materials and Methods. Flowthrough (FT) and elution (E) fractions were separated by SDS-PAGE and probed with anti-His and anti-TcpT antibodies.
