TABLE 1.
Bacterial strains and plasmidsa
| Strain or plasmid | Relevant genotype or characteristicd | Deletion or cloned endpointsb | Source or referencec |
|---|---|---|---|
| Strains | |||
| 14028 | Wild type | ATCC | |
| JS227 | ΔailT::Km | 1129388-1129938 | 22 |
| JS457 | ΔsodCI-104::Cm | 1130586-1129969 | 24 |
| JS459 | ΔsodA101 ΔsodB102 ΔsodCI-104::Cm | 24 | |
| JS460 | ΔsodA101 ΔsodB102 ΔsodCII-105::Cm | 24 | |
| JS672 | ΔsodCI::Tet | 1129358-1130612 | |
| JS673 | sodCI(H49C) ΔailT::Km | ||
| JS674 | sodCI(H49R) ΔailT::Km | ||
| JS675 | (sodCII1-25sodCI26-157)hyb ΔailT::Km | ||
| JS676 | sodCI Y87E Y107E ΔailT::Km | ||
| JS677 | ΔsodCI::sodCBa ΔailT::Km | ||
| JS678 | ΔsodA101 ΔsodB102 ΔsodCI::Tet ΔsodCII-105::Cm | ||
| JS679 | ΔsodA101 ΔsodB102 (sodCII1-25sodCI26-157)hyb ΔailT::Km ΔsodCII-105::Cm | ||
| JS680 | ΔsodA101 ΔsodB102 sodCI Y87E Y109E ΔailT::Km ΔsodCII-105::Cm | ||
| JS681 | ΔsodA101 ΔsodB102 ΔsodCI::sodCBa ΔailT::Km ΔsodCII-105::Cm | ||
| JS682 | ΔsodA101 ΔsodB102 ΔsodCI::sodCK-12 ΔailT::Km ΔsodCII-105::Cm | ||
| JS683 | ΔsodA101 ΔsodB102 ΔsodCI::sodCIII ΔailT::Km ΔsodCII-105::Cm | ||
| JS684 | ΔsodA101 ΔsodB102 ΔsodCI::sodC;Ps ΔailT::Km ΔsodCII-105::Cm | ||
| AS391 | E. coli AB1157 (F−thr-1 leuB6 proA2 his-4 thi-1 argE2 lacY1 galK2 rspL supE44 ara-14 xyl-15 mtl-1 tsx-33) (sodA::MudPR13)25 (sodB-Km)1-Δ2 sodC::Spec | 21 | |
| Plasmids | |||
| pMC101 | pWKS30 sodCI | 1129594-1130988 | |
| pRK101 | pWSK29 sodCII | 1516038-1516589 | |
| pRK104 | pWKS30 sodCI(C−5T) | ||
| pRK105 | pWKS30 sodCI(Y109E) | ||
| pRK106 | pWKS30 sodCI(Y87E Y109E) | ||
| pBK101 | pWSK29 (CI1-26CII27-153)hyb | ||
| pBK102 | pWSK29 (CII1-25CI26-157)hyb | ||
| pEM101 | pWKS30 sodCK-12 | ||
| pEM102 | pWKS30 sodCPs | ||
| pEM103 | pWKS30 sodCIII | ||
| pMEK15 | pBBR 1MCS4 sodCBa | 19 |
a
All Salmonella strains are isogenic derivatives of S. enterica serovar Typhimurium strain 14028.
b
Numbers indicate the base pairs that are deleted or cloned (inclusive) as defined for the S. enterica serovar Typhimurium LT2 genome sequence (National Center for Biotechnology Information).
c
This study, unless otherwise specified.
d
sodCK-12, sodC in E. coli K-12; sodCPs, sodC in P. syringae; sodCBa, sodC in B. abortus.