TABLE 5.

Specific activities of SodC enzymes and release by osmotic shock

Enzymee	Straina	Mean sp act ± SD (U/mg)b	% of SodC released by osmotic shockc
Multicopy enzymes in E. coli
SodCI	E. coli pMC101	69.4 ± 2.0	0.4
SodCBa	E. coli pMEK15	404.6 ± 61.9	60
SodCK-12	E. coli pEM101	51.6 ± 25.9	71
SodCIII	E. coli pEM103	241.9 ± 16.0	26
SodCPs	E. coli pEM102	5.6 ± 0.2	2
Single-copy enzymes in Salmonella
SodCI	Serovar Typhimurium	5.7 ± 1.9	6.5
SodCBa	Serovar Typhimurium	2.1 ± 0.4	109
SodCK-12	Serovar Typhimurium	0.1 ± 0.0	100
SodCIII	Serovar Typhimurium	0.2 ± 0.1	41
SodCPs	Serovar Typhimurium	BDd	BD

^aAll strains produce the indicated SOD only. Strains used were either E. coli AS391 containing the indicated plasmid or serovar Typhimurium strains JS460, JS681, JS682, JS683, and JS684.

^bSpecific activity after cell lysis by a French press (n = 2). Data are from single but repeatable experiments.

^cAs in Table 3, the amount of enzyme released by French press is considered 100%.

^dBD, below detectable level.

^eSodC_Ba, SodC in B. abortus; SodC_K-12, SodC in E. coli K-12; SodC_Ps, SodC in P. syringae.