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Alignment of a known cutinase, F. solani cut12, with M. smegmatis cutinase that we found through reverse genetics of MPLA activity and the eight putative H37Rv cutinases. Active sites (S, D, and H) are in bold; the cutinase motif is underlined. All putative cutinases except 3724a have a cutinase motif, and all except 1758 and 3724b appear to have secretion signals.
