FIG. 3.

MPLA activity determined by TLC of various mycobacterial strains. Cell pellets (P) and culture supernatants (S) were assayed using [¹⁴C]dipalmitoyl PC labeled on both palmitic acids. The presence of [¹⁴C]FA and [¹⁴C]LPC indicates hydrolysis of [¹⁴C]dipalmitoyl PC from MPLA activity. A₂, snake venom PLA₂. Culture supernatant from H37Rv is lyophilized culture filtrate (shown). Nonlyophilized H37Rv culture supernatant and nonlyophilized ammonium sulfate-precipitated H37Rv culture supernatant were also tested and were inactive; M. marinum 11566 (P+S) and M. marinum 25059 (P) were tested and were active (data not shown).