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. 2007 Apr 6;189(11):4153–4160. doi: 10.1128/JB.01909-06

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FIG. 6. — Tween hydrolysis by known cutinases and M. smegmatis partially purified MPLA assessed by TLC. The presence of oleic acid indicates hydrolysis. Lanes: OA, oleic acid; T80, Tween 80 alone; A₂, snake venom PLA₂; M, M. smegmatis partially purified MPLA; FS, F. solani cutinase (7.5 μg/ml); TL, T. lanuginosus lipase (7.5 μg/ml), all with Tween 80. M. smegmatis partially purified MPLA and F. solani and T. lanuginosis cutinases, but not snake venom PLA₂, also hydrolyzed Tween 20, Tween 40, and Tween 60 (data not shown).