TABLE 4.
Total activity and turnover ratea
| Enzyme | Total NPB activity (A410) | Turnover rate (min−1) |
|---|---|---|
| F. solani cutinase | 3.1 | 5,024 ± 77 |
| T. lanuginosus lipase | 0.43 | 916 ± 74 |
| M. smegmatis purified MPLA | 0.84 | 0.8 ± 0.1 |
a
Total absorbance and turnover rate data are averages of three independent determinations on NPB. Data are for the 60-minute time point. Protein concentrations were determined prior to dilution with a Nanodrop spectrophotometer; after dilution concentrations should be 75 ng/ml for F. solani cutinase and T. lanuginosus lipase.