TABLE 3.

qRT-PCR confirmation of microarray data

Gene product	Gene no.	Expression ratioa
		DNA microarray	Real-time PCR
Transcriptional regulator ComX1	spr0013	18.6	16.9
Hypothetical protein, LysM domain protein	spr0096	−11.0	−15.9
Sensor histidine kinase CiaH	spr0708	*	−1.6
Competence protein CelB	spr0857	2.6	22.0
Endo-beta-N-acetylglucosaminidase	spr0867	−4.8	−5.5
ABC transporter substrate binding protein-iron transport	spr0934	−2.2	−1.8
Conserved hypothetical protein, member of the hydrolase HAD-like family	spr1057	−58.5	−502.2
Formate-tetrahydrofolate ligase	spr1109	2.1	2.1
Carbamoylphosphate synthase (glutamine-hydrolyzing) light subunit	spr1154	−2.4	−3.0
Conserved hypothetical protein, similar to hemolysin III	spr1320	−10.2	−15.7
ABC transporter membrane-spanning permease-ferric iron transport	spr1684	−2.1	−10.6
Single-stranded DNA binding protein SsbB	spr1724	3.3	23.6
RecA protein	spr1757	*	1.6
Competence protein CglA	spr1864	7.6	53.7
General stress protein GSP-781	spr2021	−9.5	−7.4
Response regulator ComE	spr2041	34.7	30.1

^aThe ratio of the gene expression level in the ΔstkP mutant strain compared to that of the wild-type strain indicating genes up- or down-regulated in the ΔstkP mutant strain.

^*, gene is not significantly regulated by StkP.