TABLE 1.
Strains, plasmids, and primers
| Strain, plasmid, or primer | Genotype, description, or sequence | Source or referencea |
|---|---|---|
| Helicobacter pylori strains | ||
| 43504 (wild type) | H. pylori ATCC 43504 | ATCC |
| hpn mutant | Kanr; 43504 with hpn kanamycin resistance cassette | This study |
| hpn-like mutant | Cmr; 43504 with hpn-like chloramphenicol resistance cassette | This study |
| Double mutant | Kanr Cmr; 43504 with hpn kanamycin resistance cassette and hpn-like chloramphenicol resistance cassette | This study |
| Escherichia coli DH5α | Cloning strain | Invitrogen |
| Plasmids | ||
| pHP1 | Plasmid containing the kanamycin resistance gene aphA3 | 15 |
| pUC20cat | Plasmid containing the chloramphenicol acetyltransferase gene cat | 37 |
| pGEM-T | Ampr; cloning vector | Promega |
| pGEM-hpn | pGEM-T vector with the hpn gene from H. pylori 43504 | This study |
| pGEM-hpn-like | pGEM-T vector with the hpn-like gene from H. pylori 43504 | This study |
| pGEM-hpn: kanR | pGEM-hpn with the Kanr cassette inserted into the XcmI site of hpn | This study |
| pGEM-hpn-like: cmR | pGEM-hpn-like with the Cmr cassette inserted into the BlpI site of the hpn-like gene | This study |
| Primers | ||
| HPN-F | 5′-TGTTGCGGTTGGATTGG-3′ | IDT |
| HPN-R | 5′-CAAGTGGGTTGCTCGTTTTGTTTC-3′ | IDT |
| 1432-F | 5′-AGCCAACGCCTTTTCTTTCAG-3′ | IDT |
| 1432-R | 5′-TTTTACACCCCATTACGACCACTC-3′ | IDT |
a
IDT, Integrated DNA Technologies, Inc., Coralville, IA.