FIG. 4.

Multicolor imaging of protein synthesis patterns of S. aureus COL under anaerobic conditions in the presence and absence of nitrate. Delta 2D software was used to visualize complex protein expression patterns on the 2D image in the standard pH range of 4 to 7. The color code is shown on the right side. Proteins whose synthesis was induced under anaerobic conditions only in the presence of nitrate appear light blue, proteins whose synthesis was induced only in the absence of nitrate appear red, and proteins induced under both conditions appear yellow. In addition, proteins whose synthesis was repressed in the presence of nitrate are shown in dark blue, proteins repressed in the absence of nitrate are shown in green, and proteins repressed under both conditions are shown in brown.