TABLE 1.
Oligonucleotides used in this study
| Gene identifiera | Gene namea | Primer
|
|
|---|---|---|---|
| Direction | Sequence (5′ to 3′)b | ||
| SACOL0222 | ldh1 | Forward | CATGCCACACCATATTCTCC |
| Reverse | CTAATACGACTCACTATAGGGAGATCTGCTTCAGCCATAATATC | ||
| SACOL0301 | Forward | GGACGACTGGGTAAATAACG | |
| Reverse | CTAATACGACTCACTATAGGGAGAGGCGAATATGGTAACACCGT | ||
| SACOL0838 | gapA1 | Forward | GTTTCACAGGTGAAGTAGAGG |
| Reverse | CTAATACGACTCACTATAGGGAGAACACGAGTTTGTGTAGCGTC | ||
| SACOL1746 | pfkA | Forward | ACTAGTGGTGGAGATTCACC |
| Reverse | CTAATACGACTCACTATAGGGAGAACCAACTGATAATCCAGCCC | ||
| SACOL2363 | Forward | GTATCGCTTCTGGTGCAGTA | |
| Reverse | CTAATACGACTCACTATAGGGAGACTGCTAATTCAGGTCCACTG | ||
| SACOL2399 | nirR | Forward | TTGTTGCACATGGCATGAGG |
| Reverse | CTAATACGACTCACTATAGGGAGATTCTCGCATATGTCGGCTTG | ||
a
Based on TIGR annotation (http://www.tigr.org).
b
The underlined sequences at the 5′ end represent the recognition sequence for the T7 RNA polymerase (33).