FIG. 1.

Gene organization and regulation of znuACB and zur loci in S. coelicolor. (A) Comparison of gene organization patterns of znuACB loci in S. coelicolor and E. coli. (B) Regulation of znuA and zur genes by Zur. Transcripts from znuA and zur genes were analyzed by S1 nuclease mapping. RNAs were prepared from the wild-type (WT) and Δzur (S701) strains grown in YEME complex medium. To chelate metals, EDTA at a 2 mM final concentration was added for 1 h before cell harvesting. For each S1 analysis, the same amount of RNA (50 μg) was used and the amount was monitored by using rRNAs. +, present; −, absent. (C) Transcription start sites and predicted promoter elements for the znuA and zur genes. Transcription start sites (+1) were determined by high-resolution S1 mapping analyses. Predicted −10 and −35 elements are shown in bold characters and underlined.