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. 2007 Mar 23;189(11):4320–4324. doi: 10.1128/JB.00003-07

FIG. 4.

FIG. 4.

Comparison of reversed-phase HPLC profiles of intracellular and extracellular acyl-HSLs produced by the B. pseudomallei bpeR null mutant and wild-type KHW carrying a plasmid overexpressing the BpeR repressor. (A) [14C]acyl-HSLs extracted from supernatants of untreated stationary-phase bacterial cultures. (B) [14C]acyl-HSLs extracted from bacterial cells permeabilized by repeated freeze-thawing in fresh AB medium. Peak positions 1, 2, 3, 4, 5, 6, and 7 correspond to the elution profiles of methionine, C8HSL, C10HSL, 3-hydroxy-C8HSL, 3-oxo-C10HSL, 3-hydroxy-C10HSL, and 3-oxo-C14HSL, respectively. Both the bpeR null mutant and the wild-type KHW carrying a plasmid overexpressing the BpeR repressor failed to synthesize any 3-oxo-C14HSL (peak 7). The HPLC profiles of acyl-HSLs extracted from culture supernatants of KHW bpeR and KHW(pUCP28TbpeR) are represented by black circles and gray circles, respectively.