TABLE 1.
Bacterial strains used in this study
| Strain | Characteristicsa | Source or reference |
|---|---|---|
| E. coli strainsb | ||
| DH5α | endA hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15] | Gibco-BRL |
| JB525 | Indicator bacterium used for detecting acyl-HSLs; derivative of E. coli MC1000 harboring plasmid pJBA132 Tetr | 2 |
| B. pseudomallei strains | ||
| KHW | Wild-type parental strain; clinical isolate | 5 |
| KHWΔbpeAB | bpeAB deletion mutant derived from KHW; Kanr | 4 |
| KHWΔbpeAB(pUCP28TbpeAB) | bpeAB deletion mutant harboring pUCP28T carrying full-length bpeAB; Kanr Tmpr | 4 |
| KHWbpeR::Km | bpeR null mutant derived from KHW by insertion of a kanamycin resistance cassette into bpeR; Kanr | 4 |
| KHW(pUCP28TbpeR) | Wild-type strain overexpressing bpeR; KHW harboring pUCP28T carrying full-length bpeR; Tmpr | 3 |
a
Kan, kanamycin; Tmp, trimethoprim; Tet, tetracycline; Amp, ampicillin.
b
E. coli strains were cultured in LB broth and maintained on LB agar (Becton Dickinson, Cockeysville, MD).