TABLE 4.

SYBR green quantitative RT-PCR results for select genes identified as significantly increased in expression by SAM

Genea	Fold change in gene expressionb by SYBR green quantitative RT-PCR for the following strain(s) and conditionc:
	Sterne + PQ	ΔsodA1 mutant + PQ	ΔsodA1 mutant in LB vs Sterne in LB
entA-dhbC	+24	+34	+7
asbA	+4	+10	−2
Glyoxalase family	+58	+27	0
ccdA-1	NPd	NP	+49
Thioredoxin family	NP	NP	+79
Prolipoprotein	NP	NP	+11

^aGene-specific primers amplify the following regions: for entA-dhbC, the overlapping region across the first two genes of the bac operon, GBAA2368 and GBAA2369; for asbA, the second gene in the asb operon (petrobactin biosynthetic genes), GBAA1982; for the glyoxalase family protein, GBAA3339; for ccdA-1, the cytochrome c-type biogenesis protein gene GBAA1778; for the thioredoxin family protein, GBAA1779; and for the prolipoprotein diacylglyceryl transferase family protein, GBAA1780.

^bNormalized against expression by the fusA constitutive control. C_T values for fusA had a standard deviation of <1 cycle for all RNA samples (see Materials and Methods), ranging from 12.19 to 12.83.

^cPQ, paraquat (800 μM).

^dNP, not performed. These genes were not quantified by SYBR green quantitative RT-PCR for paraquat-treated samples.