TABLE 2.
Comparison of 1,3-poly(glycerophosphate) backbones of different LTAsa
Strain | LTA fraction | Chain length [(Gro-P)n] | Chain substitution (%)
|
||
---|---|---|---|---|---|
d-Ala | α-Glc | None | |||
ATCC 15808 | 1 | 37 | 27 | 3 | 70 |
2 | 29 | 21 | 3 | 76 | |
Ads-5 | 1 | 36 | 42 | ND | 58 |
2 | 31 | 49 | ND | 51 | |
ATCC 15808(J1)b | 1 | 32 | ND | 68 | |
2 | 54 | ND | 46 | ||
LL78 | 1 | 30 | 28 | 27 | 45 |
2 | 34 | 25 | 26 | 49 |
LTAs were extracted with hot aqueous phenol and purified by HIC from different L. delbrueckii subsp. lactis strains. Unless stated otherwise, the values are based on NMR analyses. ND, not detected.
Polyols and α-glucose were quantified by GLC, and amino acids were quantified by the use of an amino acid analyzer.