FIG. 2.

Construction and characterization of the M. tuberculosis H37Rv Rv2953::Km mutant strain. (A) Schematic diagram of the genomic organization of the Rv2953 locus in the wild-type strain of M. tuberculosis H37Rv and the PMM80 (Rv2953::Km) mutant. The black box represents the Rv2953 gene, and the hatched box represents the fragment deleted during the construction of the knockout mutant. The Km resistance cassette used for targeted disruption is represented by a gray box. Names of primers are indicated by letters (A, 2953A; B, 2953B; C, 2953C; D, 2953D; E, 2953E; res1; and res2). Positions are indicated by arrowheads below each genetic structure, and the expected sizes for PCR products are indicated. (B) PCR analysis of the recombinant strain PMM80 using various combinations of specific primers.