Alignment of YoeB from B. subtilis (Bsu), B. amyloliquefaciens (Bam), and B. licheniformis (Bli). The predicted signal sequence cleavage site is after Ala23, as shown by the black triangle. Boxed sequences are an alignment of partial sequences from S. sciuri (Ssc) PBP2a (aa 73 to 131 of 665), S. sciuri MecA1 (aa 61 to 131 of 666), and B. subtilis (Bsu) PbpC (aa 64 to 140 of 668). Black shading of nonboxed sequences, conserved in all three YoeB proteins; black shading of boxed sequences, conserved in all six proteins; dark gray shading of nonboxed sequences, conserved in two out of three YoeB proteins; light gray shading of boxed sequences, conserved in at least four of the six proteins. Alignments were performed with ClustalW and shaded with the Genedoc program (http://www.psc.edu/biomed/genedoc).