FIG. 1.
Mutant ΔsbcD, ΔsbcC, and ΔsbcCD strains of bacteria show an increased sensitivity to mitomycin C treatment and to γ-irradiation. Strain GY9613 (wild type, filled squares), GY12908 (ΔsbcD, filled inverted triangles), GY12909 (ΔsbcC, filled diamonds), and GY12910 (ΔsbcCD, filled triangles) bacteria grown to an A650 of 1.5 were exposed to mitomycin C (A) or γ-irradiation (B) at doses indicated on the abscissa. Strains GY12936 (wild type/p11520, squares), GY12923 (ΔsbcCD/p11520, triangle), and GY12912 (ΔsbcCD/p13002, sbcCD+, circles) were also exposed to γ-irradiation (C). Cell survival was measured as described in Materials and Methods.