TABLE 2.
Expression data obtained in the study
| Straina | Rapamycin expression
|
Red pigment production
|
||
|---|---|---|---|---|
| Avg % change (SD)b | No. of isolates/no. of expts | Avg rppA optical density at 488 nm (SD) | No. of isolates/no. of expts | |
| wt | 100 (2.3-8.1) | 21/5 | 0.013 (0.02) | 4/1 |
| wt pGP9 | 104 (18) | 5/1 | ||
| wt rapH | 127-155 (1.6-9.3) | 23/3 | ||
| wt rapG | 120-132 (2.4-9.3) | 12/2 | ||
| wt rapHG-np | 140 (9.3) | 3/1 | ||
| wt rapHG | 102 (8.1) | 4/1 | ||
| ΔHG | 0 (0) | 7/3 | ||
| ΔHG rapH | 0 (0) | 16/2 | ||
| ΔHG rapG | 6-13 (2.1-5.1) | 18/3 | ||
| ΔHG rapHG-np | 72-93 (2.7-5.7) | 18/3 | ||
| ΔHG rapHG | 3-16 (1.9-3.3) | 12/2 | ||
| wt rppA-PrapA | 1.244 (0.3) | 9/1 | ||
| ΔHG rppA-PrapA | 0.516 (0.1) | 9/1 | ||
| wt rppA-PactI | 0.246 (0.06) | 10/1 | ||
| wt rppA-ErmE* | 1.766 (0.26) | 6/1 | ||
a
wt, wild-type S. hygroscopicus NRRL 5491; ΔHG, deletion strain in which rapH and rapG were removed. np, gene under the control of the native promoter.
b
Each isolate was run in triplicate. Values were calculated with SAS/STAT, using means and the univariate procedure to test the normality of distribution. Using the GLM model, data were calculated as least mean square and are presented as an average change observed from all experiments when comparing least mean square values to the wild-type control least mean square value of each experiment.