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SDS-PAGE of the membrane fraction of G. metallireducens grown on different substrates. Extracts from cells grown on p-cresol (lane 1), p-hydroxybenzoate (lane 2), benzoate (lane 3), and acetate (lane 4) are shown. Spots A to C were analyzed by mass spectrometry and identified as two isoforms of the FAD-containing α subunit of p-cresol methylhydroxylase (A and B) and as a hypothetical protein, Gmet_2148 (C). Lane M, molecular size marker.
