TABLE 2.
Gene clusters involved in p-cresol and p-hydroxybenzoate metabolism of G. metallireducensa
| GI no. | Name | Annotation | Molecular mass (Da) | Induction of mRNA by cells grown on:
|
||||
|---|---|---|---|---|---|---|---|---|
| p-Cresol | 4-OH-Benzoate | Benzoate | Butyrate | Acetate | ||||
| 78223324 | pcmA | Hypothetical membrane protein | 41,805 | ++ | ++ | − | − | − |
| 78223325 | pcmB | FAD-dependent oxidoreductase | 65,958 | ++ | ++ | − | − | − |
| 78223326 | pcmC | Cytochrome bc-like, N terminal | 27,911 | ++ | ++ | − | − | − |
| 78223327 | pcmD | Cytochrome bc-like, C terminal | 19,230 | ND | ND | ND | ND | ND |
| 78223328 | pcmE | Rieske protein, [2Fe-2S] binding | 18,459 | ND | ND | ND | ND | ND |
| 78223329 | pcmF | Cytochrome c, class I | 35,417 | ++ | ++ | − | − | − |
| 78223330 | pcmG | Cytochrome c with twin arginine translocation motif | 7,563 | ND | ND | ND | ND | ND |
| 78223331 | pcmH | Hypothetical protein | 14,828 | ++ | +++ | − | − | − |
| 78223332 | pcmI | p-Cresol methylhydroxylase, FAD containing | 59,372 | ++ | ++ | − | − | − |
| 78223333 | pcmJ | p-Cresol methylhydroxylase, FAD containing | 57,029 | ++ | + | − | − | − |
| 78223334 | pcmK | Phenol degradation-like protein | 27,258 | ND | ND | ND | ND | ND |
| 78223335 | pcmL | Acetate and butyrate kinase | 38,949 | ND | ND | ND | ND | ND |
| 78223336 | pcmM | Short-chain dehydrogenase | 26,687 | ND | ND | ND | ND | ND |
| 78223337 | pcmN | Pyridoxamine 5′-phosphate oxidase-related, FMN binding protein | 15,260 | ND | ND | ND | ND | ND |
| 78223338 | pcmO | Aldehyde dehydrogenase | 51,623 | ++ | ++ | − | − | − |
| 78223339 | pcmP | Twin-arginine translocation protein TatA/E | 7,153 | ND | ND | ND | ND | ND |
| 78223340 | pcmQ | Transcriptional regulator, MarR family | 15,388 | ++ | +++ | ++ | (+) | (+) |
| 78223341 | pcmR | Molybdopterin dehydrogenase, FAD binding | 30,815 | +++ | +++ | +++ | (+) | (+) |
| 78223342 | pcmS | Molybdenum dehydrogenase, Ferredoxin [2Fe-2S] binding protein | 17,852 | ND | ND | ND | ND | ND |
| 78223343 | pcmT | Aldehyde oxidase and xanthine dehydrogenase, molybdopterin binding | 84,541 | ++ | ++ | ++ | (+) | (+) |
| 78223344 | pcmU | XdhC-like, xanthine and CO dehydrogenase maturation factor | 28,018 | + | + | + | − | − |
| 78223345 | pcmV | XdhC-like, xanthine and CO dehydrogenase maturation factor | 28,265 | + | + | + | − | − |
| 78223346 | pcmW | MobA-related protein | 27,544 | ND | ND | ND | ND | ND |
| 78223347 | pcmX | Conserved hypothetical protein, possibly involved in molybdenum cofactor biosynthesis | 21,905 | ND | ND | ND | ND | ND |
| 78223348 | blac | β-Lactamase like | 39,903 | +/− | +/− | +/− | +/− | +/− |
| 78223349 | act | GCN5-related N-acetyltransferase, acetyl-CoA hydrolase/transferase | 69,020 | +/− | +/− | +/− | +/− | +/− |
| 78223350 | bamY | Benzoate-CoA ligase family | 58,412 | +++ | ++ | +++ | − | − |
| 78223351 | bamX | Hypothetical protein | 46,310 | + | + | + | − | − |
| 78223352 | bamW | Two-component, sigma 54-specific, transcriptional regulator, Fis family | 49,891 | + | + | + | − | − |
a
The gene products identified by proteome analysis are shown in boldface. The annotation is taken from the NCBI gene bank (NZ_AAAS00000000). ND, not determined.