TABLE 1.
B. subtilis strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| 168 | Wild type, prototroph | Laboratory stock |
| 168trp | trpC2 | Alison Hunt |
| ALMT | purB6 leuA8 metB5 thr-5 | 6 |
| ALMTstr | purB6 leuA8 metB5 thr-6 strA1 | 6 |
| 168 ΔybxF cat | ΔybxF Cmr | This study |
| 168 ΔybxF cat str | ΔybxF Cmr Strr | This study |
| ALMT ΔybxF cat | purB6 leuA8 metB5 thr-5 ΔybxF Cmr | This study |
| ALMT ΔybxF cat str | purB6 leuA8 metB5 thr-5 ΔybxF Cmr Strr | This study |
| Co1 | amyE::cat Cmr | This study |
| Co2 | purB6 leuA8 metB5 thr-5 amyE::cat Cmr | This study |
| Co3 | purB6 leuA8 metB5 thr-5 strA1 amyE::cat Cmr Strr | This study |
| 168 ΔymxC spc | ΔymxC Spcr | This study |
| 168 ΔybxF cat ΔymxC spc | ΔybxF Cmr ΔymxC Spcr | This study |
| I (ybxF+ybxF-gfp) | trpC2 ybxF+amyE::ybxF-gfp Spcr | This study |
| II (ΔybxF ybxF-gfp) | trpC2 ΔybxF CmramyE::ybxF-gfp Spcr | This study |
| III (ybxF+gfp) | trpC2 ybxF+amyE::gfp Spcr | This study |
| IV (ΔybxF gfp) | trpC2 ΔybxF CmramyE::gfp Spcr | This study |
| V (ΔybxF ybxF K17A-gfp) | trpC2 ΔybxF CmramyE::ybxF K17A-gfp Spcr | This study |
| VI (ΔybxF ybxF K21A-gfp) | trpC2 ΔybxF CmramyE::ybxF K21A-gfp Spcr | This study |
| VII (ΔybxF ybxF K24A-gfp) | trpC2 ΔybxF CmramyE::ybxF K24A-gfp Spcr | This study |
| Plasmids | ||
| pUC18 | Original vector used for knockout construct preparation (2,686 bp) | Commercial source |
| pDG1728 | Original vector used for spc gene PCR amplification (10,776 bp) | 7 |
| pCPP-31 | Original vector used for knockout construct preparation (5,620 bp) | 17 |
| pGEX-5X3 | Original vector used for knock-out construct preparation (4,974 bp) | Commercial source |
| pDG1662 | Integrational vector containing chloramphenicol acetyltransferase cassette (6,982 bp) | 7 |
| pYBXFK | pUC18 containing ybxF knockout construct (5,458 bp) | This study |
| pYBXFKs | pUC18 containing ybxF knockout construct − rpsL including the strA1 mutation (5,458 bp) | This study, reference 12 |
| pYMXCK | pGEX-5X3 containing the ymxC knockout construct (7,759 bp) | This study |
| pSG1154 | GFP-fusion vector containing the GFP cassette (7,600 bp) | 19 |
| pSG1154F11 | pSG1154 containing the ybxF gene (7,843 bp) | This study |
| pSG1154F11_K17A | pSG1154 containing the mutated ybxF gene (7,843 bp) | This study |
| pSG1154F11_K21A | pSG1154 containing the mutated ybxF gene (7,843 bp) | This study |
| pSG1154F11_K24A | pSG1154 containing the mutated ybxF gene (7,843 bp) | This study |