FIG. 3.

CFU of C. glutamicum parental strain RES167 and sigM mutant C. glutamicum DN1 after the application of different stress conditions. Cultures of C. glutamicum RES167 and DN1 were grown in minimal medium (MM1), and cells were harvested during exponential growth at an optical density of 7. The following stress conditions were applied for 15 min: heat shock at 50°C, cold shock at 10°C, disulfide stress caused by 2 or 10 mM diamide, oxidative stress caused by 1% hydrogen peroxide, salt stress caused by 1 M NaCl, and alcohol stress caused by 10% ethanol. Following stress treatment, the colony-forming ability of the cultures was determined with three biological and two technical replicates. CFU percentages are represented by black (RES167) and gray (DN1) columns.