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. 2007 May 4;189(13):4696–4707. doi: 10.1128/JB.00382-07

FIG. 6.

FIG. 6.

Promoter sequences of disulfide stress-responsive genes. (A) Alignment of SigM-dependent promoter regions of differentially expressed genes of C. glutamicum RES167. The transcriptional start sites (+1) were determined by RACE-PCR, with total RNA from a C. glutamicum RES167 culture treated with diamide. sigR (Sc), consensus sequence of SigR-dependent promoters from S. coelicolor (50). (B) Promoter sequences of other C. glutamicum genes potentially transcribed with the help of SigM. The promoter regions (−10 and −35) were detected by similarities to the consensus sequence shown in panel A. (C) Nucleotide sequence of the suf operon promoter region of C. glutamicum. The transcriptional start sites (+1) and deduced promoter elements (−10 and −35) of the housekeeping promoter (underlined) and the stress-responsive SigM promoter (overlined) were determined by RACE-PCR. (D) Mapped promoter of the sigM gene of C. glutamicum. sigH (Mt), consensus sequence of SigH-dependent promoters from M. tuberculosis (33). Abbreviations: R, A or G; Y, C or T.