FIG. 3.

Growth and enzyme properties of wild-type and mutant strains. (a) Growth profiles of wild-type (diamonds), sboR disruptant (squares), and sboA disruptant (triangles) strains grown on different C sources, including 1% d-sorbitol (left), 0.5% l-sorbose (middle), and 1% glycerol (right). (b) Enzyme activities in soluble fractions from each mutant strain grown on different C sources. NADPH-SR activity (hatched bars) was measured in 50 mM KPB (pH 6.0), and NAD-SLDH activity (gray bars) was measured in 100 mM Tris-HCl (pH 9.0). All assays were performed in triplicate, and the means and standard deviations are shown.