TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| Strains | ||
| G. frateurii strains | ||
| THD32 | Wild-type strain; NBRC 101656 | 25 |
| sboR::Tcr | THD32 defective in sboR by insertion of Tcr cassette | This study |
| sboA::Tcr | THD32 defective in sboA by insertion of Tcr cassette | This study |
| sboR::Tcr(pSAsboR) | sboR disruptant harboring the plasmid pSAsboR | This study |
| sboA::Tcr(pSABsboA) | sboA disruptant harboring the plasmid pSABsboA | This study |
| sboA::Tcr(pSAEsboA) | sboA disruptant harboring the plasmid pSAEsboA | This study |
| E. coli strains | ||
| DH5α | F−endA1 hsdR17 recA1 supE44 thi-1 φ80dlacZΔM15 | Toyobo |
| BL21(DE3) | F−dcm ompT hsdSB(rB− mB−) gal | Novagen |
| Plasmids | ||
| pGEM-T Easy vector | E. coli cloning vector; Ampr | Promega |
| pUC119 | E. coli cloning vector; Ampr | 29 |
| pKRP12 | Tetracycline cassette; Tcr | 10 |
| pET-28a(+) | E. coli T7 system expression vector; Kmr | Novagen |
| pSA19 | Gluconobacter cloning vector; Ampr | 24 |
| pUC46 | Carries 3.5-kb DNA fragment at SphI site of pUC119; Ampr | This study |
| pSAsboR | Carries 0.8 kb of sboA at SmaI and SalI sites of pSA19; Ampr | This study |
| pUCSDRTc | Insertion of Tcr cassette at HincII site of pUCSDR; Tcr Ampr | This study |
| pUCsboRTc | Insertion of Tcr cassette at HincII site of pUCsboR; Tcr Ampr | This study |
| pETsboA | pET-28a(+) with the complete coding sequence of sboA | This study |
| pSABsboA | pSA19 with the complete coding sequence of sboA downstream of the lac promoter | This study |
| pSAEsboA | pSA19 with the complete coding sequence of sboA in the opposite direction from that of the lac promoter | This study |