FIG. 7.

Quantitative coagulation assay of S. aureus in the presence of oligosaccharides from GXM of C. neoformans. (A and B) Cells of S. aureus were incubated with 100 μM (A) or 500 μM (B) of the oligosaccharides. C, control (without saccharide); M1, mannose; M2, mannobiose. (C) Cells of S. aureus were incubated with 0, 10, 100, 500, and 1,000 μM of trisaccharide. After incubation overnight at 37°C, the number of clumps per milliliter that were larger than about 1/16 mm was determined with a microscope. Values that are significantly different (P < 0.05) from the control value (A and B) or the no-mannotriose value (C) are indicated (*).