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. 2007 Apr 27;189(13):4774–4783. doi: 10.1128/JB.00143-07

TABLE 1.

Primers used for CttA expression

Primer Sequencea Purpose
His6-CttA forward GACGACGACAAGATGGCTGCTGGTCAGGCTTATG Cloning of His6-CttA
His6-CttA reverse GAGGAGAAGCCCGGTTTAAGATGTAGTACTCTCAACCTG Cloning of His6-CttA
His6-CttA forward GACGACGACAAGATGGCTGCTGGTCA GGCTTATG Cloning of 2X-His6-CttA
His6-CttA reverse GAGGAGAAGCCCGGTTTACACCACCACCACCACCACAGATGTAGTACTCTCAACCTG Cloning of 2X-His6-CttA
CttAα1 forward GACGACGACAAGATGGCTGCTGGTCAGGCTTATG Cloning of CttAα1
CttAα1 reverse GAGGAGAAGCCCGGTTTAGCCTTGAGCCAGCCATTTG Cloning of CttAα1
CttAα2 forward GACGACGACAAGATGGAAGTTGCCGCTGATAAGGTT Cloning of CttAα2
CttAα2 reverse GAGGAGAAGCCCGGTTTAAGATGTAGTACTCTCAACCTG Cloning of CttAα2
XynDoc forward CGCTGGTACCTGCTAACTACGATCACTCCTAC Cloning of XynT6-XDoc fusion protein
XynDoc reverse GGACAGATCTTATTTACCGAATCTTGCGTC Cloning of XynT6-XDoc fusion protein
CBM-Coh forward GCTAGGATCCGGTCAGGCTTATGATGC Cloning of CBM3-Coh fusion protein
CBM-Coh reverse GGCCGCTCGAGTTAAGATGTAGTACTCTC Cloning of CBM3-Coh fusion protein
a

Underlined sections are specific for the LIC site in the vector, the stop codon is highlighted in bold, and nucleotides coding for a second His6 tag are shown in italics.