TABLE 1.
Primers used for CttA expression
| Primer | Sequencea | Purpose |
|---|---|---|
| His6-CttA forward | GACGACGACAAGATGGCTGCTGGTCAGGCTTATG | Cloning of His6-CttA |
| His6-CttA reverse | GAGGAGAAGCCCGGTTTAAGATGTAGTACTCTCAACCTG | Cloning of His6-CttA |
| His6-CttA forward | GACGACGACAAGATGGCTGCTGGTCA GGCTTATG | Cloning of 2X-His6-CttA |
| His6-CttA reverse | GAGGAGAAGCCCGGTTTACACCACCACCACCACCACAGATGTAGTACTCTCAACCTG | Cloning of 2X-His6-CttA |
| CttAα1 forward | GACGACGACAAGATGGCTGCTGGTCAGGCTTATG | Cloning of CttAα1 |
| CttAα1 reverse | GAGGAGAAGCCCGGTTTAGCCTTGAGCCAGCCATTTG | Cloning of CttAα1 |
| CttAα2 forward | GACGACGACAAGATGGAAGTTGCCGCTGATAAGGTT | Cloning of CttAα2 |
| CttAα2 reverse | GAGGAGAAGCCCGGTTTAAGATGTAGTACTCTCAACCTG | Cloning of CttAα2 |
| XynDoc forward | CGCTGGTACCTGCTAACTACGATCACTCCTAC | Cloning of XynT6-XDoc fusion protein |
| XynDoc reverse | GGACAGATCTTATTTACCGAATCTTGCGTC | Cloning of XynT6-XDoc fusion protein |
| CBM-Coh forward | GCTAGGATCCGGTCAGGCTTATGATGC | Cloning of CBM3-Coh fusion protein |
| CBM-Coh reverse | GGCCGCTCGAGTTAAGATGTAGTACTCTC | Cloning of CBM3-Coh fusion protein |
a
Underlined sections are specific for the LIC site in the vector, the stop codon is highlighted in bold, and nucleotides coding for a second His6 tag are shown in italics.