FIG. 1.

Deletion of the dsbA gene affects neither the growth of bacteria nor yihE expression in the LF82-ΔdsbA mutant. (A) Growth of the wild-type strain LF82 and of LF82-ΔdsbA in RPMI medium supplemented with 10% heat-inactivated FCS. (B) Analysis of yihE mRNAs of LF82 and LF82-ΔdsbA by two-step RT-PCR using primers annealing the 5′and 3′ ends of the yihE cDNA. (C) yihE mRNA level of LF82-ΔdsbA relative to that of the wild-type strain LF82 determined by real-time RT-PCR experiments after growth of the bacteria in LB medium. Only experiments showing the same levels of 16S rRNA for each sample were taken into account.