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TEM of gold immunolabeling of LF82 bateria (A), LF82-ΔdsbA (B), and LF82-ΔdsbA transformed with plasmid pPBI11 harboring cloned dsbA (C), with plasmid pPBI01 harboring the cloned E. coli K-12 fim operon (D), or with cosmid JE7 harboring the cloned LF82 fim operon (E) using polyclonal antibodies raised against purified type 1 pili. Arrows indicate a flagellar structure. Bars, 0.5 μm.
