TABLE 2.
Oligonucleotides and PCR product sizes
| Primer | Oligonucleotide sequence (5′→3′) | PCR product size (bp) | Use |
|---|---|---|---|
| IMdsbA-1 | AGAACCCCCTTTGCAATTAACACCTATGTATTAATCGGAGAGAGTAGATCGTAGGCTGGAGCTGCTTCG | 1,573 | LF82-ΔdsbA isogenic mutant construction |
| IMdsbA-2 | TAATAAAAAAGCCCGTGAATACTCACGGGCTTTATGTAATTTACATTGAACATATGAATATCCTCCTTAGTTC | ||
| DsbA-1 | CTGCCGGAAGGCGTGAAC | 237 | Allelic replacement of dsbA in LF82-ΔdsbA and RT-PCR |
| DsbA-2 | GCTGTTCCACGCCGCGTC | ||
| HindIII-dsbA | CCCAAGCTTGGGGTGAATACTCACGGGCTTTAAG | 706 | Transcomplementation of LF82-ΔdsbA |
| XbaI-dsbA | CCCGGGTCTAGATAATCGGAGAGAGTAGATCATG | ||
| k1 | CAGTCATAGCCGAATAGCCT | Kmr cassette amplification (20) | |
| k2 | CGGTGCCCTGAATGAACTGC | ||
| kt | CGGCCACAGTCGATGAATCC | ||
| FIME | GCAGGCGGTTTCTTACGGGG | 750 | OFF-oriented invertible element |
| INV | GAGGTGATGTGAAATTAATTTAC | ||
| FIMA | GATGCGGTACGAACCTGTCC | 450 | ON-oriented invertible element |
| INV | GAGGTGATGTGAAATTAATTTAC | ||
| htrA-1 | GCGAACTGATCGGTATCAAC | 168 | qRT-PCR |
| htrA-2 | GAGTTCAGCTCAGTCCCCA | ||
| yihE3 | CGCCCTGAACGTTGGACAGC | 208 | qRT-PCR |
| yihE4 | CGCCCAACCGCTTCCATCTG | ||
| yihE3 | CGCCCTGAACGTTGGACAGC | 822 | RT-PCR |
| yihE5 | CTACTCTCTCCGATTAATACATAG | ||
| 16S-1 | ATGACCAGCCACACTGGAAC | 157 | qRT-PCR |
| 16S-2 | CTTCCTCCCCGCTGAAAGTA |