FIG. 7.

Weight matrices demonstrating natural variation in ComK-regulated K-boxes. (A) Weight matrix based on the sequences of 88 ComK-regulated K-boxes. On the x axis, the two AT-boxes, composing a K-box, are shown. In order to align the AT-boxes for the three classes of K-boxes, the spacer was in all cases set to the same length. For each position in the AT-boxes, which by consensus contain four A's and four T's, the percentage of occurrence of each of the four possible nucleotides among the 88 ComK-regulated K-boxes was calculated. This percentage is shown on the y axis. (B) Weight matrix based on the sequence of 11 out of the 88 ComK-regulated K-boxes, which contain a T-N substitution at position 2 in AT-box 1.