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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Int Immunopharmacol. 2006 Nov 20;7(3):277–286. doi: 10.1016/j.intimp.2006.10.006

Fig 8.

Fig 8

Effect of histamine on phosphorylation of STAT6 in the presence of anti-IL-4 and anti-IL-13. C57BL/6 splenocytes were pre-treated with anti-IL-4 and anti-IL-13 for 30 min followed by treatment with or without histamine (10−5 M & 10−11M) for 1 h. The cells were then stimulated with PMA + ionomycin (10 ng/ml and 1 ug/ml, respectively) for 6 h and subsequently lysed. The levels of STAT6 phosphorylation were determined by Western Blot Analysis. a. Lane 1: untreated; lane 2: PMA + ionomycin; lane 3: PMA + ionomycin + histamine 10−5 M; lane 4: PMA + ionomycin + histamine 10−5 M + anti-IL-4 + anti IL-13; lane 5: PMA + ionomycin + anti-IL-4 + anti IL-13. b. lane 1: untreated; lane 2: PMA + ionomycin; lane 3: PMA + ionomycin + histamine 10−11 M; lane 4: PMA + ionomycin + histamine 10−11 M + anti-IL-4 + anti IL-13; lane 5: PMA + ionomycin + anti-IL-4 + anti IL-13.