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. 2007 Jun 21;5:27. doi: 10.1186/1741-7007-5-27

Figure 1.

Figure 1

This is the original image for figure 2, with the corrected version of the legend. Elevated nucleotide turnover by Y831A Pol ε. DNA synthesis and dTTP turnover by Pol ε was measured on poly(dA)/oligo(dT) substrate. Reactions were performed as described in Methods, using 0.13 U of each enzyme for 20 μl reactions. (A) Analysis of polymerase reaction by TLC in 1 M LiCl running buffer. Lanes 1, 2, 3, 4: wild-type Pol ε at 0, 3, 7 and 15 minutes of reaction, respectively. Lanes 5, 6, 7: reactions with Y831A Pol ε at 3, 7 and 15 minutes, respectively. Positions of unincorporated label, label in DNA and dTMP are shown by arrows. (B) Analysis of polymerase reaction by TLC in 0.4 M LiCl running buffer. Lane assignment is the same as in (A). (C) Plot of time-course of DNA synthesis by wild-type Pol e (open circles connected by solid line) and Y831A Pol e (open rectangles connected by a dashed line). The data are derived from Fig 2A and are based on amount of dTMP incorporated into DNA. (D) Plot of time-course of dTMP generation by 3'->5' exonuclease activity by wild-type Pol e and Y831A Pol e (symbols are the same as in (C)). The data are derived from Fig 2B and based on amount of dTMP excised from DNA (turnover).