Table 1.
Frequency of activation of in vitro maturing oocytes which were fertilised, during maturation, by ISCI, 1 – 4 h after GVBD, refertilized at metaphase II (ICSI + IVF) and were fixed for cytological examination 7 – 8 h after refertilisation. Control, in vitro maturing oocytes were inseminated once: only by ICSI during maturation (ICSI), only in MII stage (IVF) or were not inseminated at all (no ICSI and no IVF).
Proportion (%) of oocytes with | ||
2 PB | pronuclei | |
ICSI + IVF | 29/39 (78)a | 27/31 (87)c |
IVF | 35/37 (95)a | 25/27 (93)c |
ICSI | 0/27 (0)b | 3/27 (11)d |
No ICSI and no IVF | 0/45 (0)b | 2/37 (5)d |
a, b and c, d Values with different superscripts are significantly different (p < 0.05).