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Agar-spot tests showing antibacterial activity of synthetic Pep13. Indicator strains were grown in LB to an OD600= 0.6 and diluted to OD= 0.2 before spreading on LB agar. We spotted 15 μl of Pep13, at dilutions of 7.26 mM to 0.93 mM, on indicator strains. Antibacterial activity was assessed after overnight incubation at 37°C.
