Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used
| Primer name | 5'-3' sequence* | Restriction sites |
| Ppep1-L | GATACTGCAGCCTTATGGGCCAATAGCAGT | PstI |
| Ppep1-R | CGTCGGATCCTGATTGATAAATGATTGCTAACTAA | BamHI |
| Ppep2-L | CGCAAGCTTTCTAAACAAGGAATCCTACAAAG | HindIII |
| Ppep2-R | CGCGGATCCCTCCTCTTTTTCGTATTAAGATG | BamHI |
| Ppep3-L | CGCAAGCTTGGAAATAGTGGGTCTAGAACAT | HindIII |
| Ppep3-R | CGCGGATCCCCTCTTTTGTTAATACTGGGA | BamHI |
| Extsnclo | CTAACTAATAAACATGCAAGGAAC |
* Restriction enzyme sites are underlined.
