Table 3.
Selected Opsithorchis viverrini contigs that encode families of secreted/membrane proteins that potentially interact with or are exposed to host tissues. Genera of the closest homologues from BLAST × (nr) searches are shown. Where the closest homologue was from a vertebrate (bold font), a tBLASTx search against dbEST was conducted.
| Predicted function | Examples/comments and genera of closest orthologues/paralogues | Contigs | %identities |
| TGF-β receptor | bone morphogenic protein receptor type I (Sus nr/Macaca est) | OvAE22 | 44 |
| Seven transmembrane receptor | DC-STAMP (Strongylocentrotus); laminin receptor (Bos nr/Clonorchis est) | OvAE92, OvAE1722 | 51 |
| Tetraspanin | stabilize cell membranes – expressed in the tegument of schistosomes (Schistosoma) | OvAE953 | 34 |
| C1 family papain-like cysteine protease | cathepsin L (Paragonimus), cathepsin B (Fasciola; Clonorchis) | OvAE1795, OvAE813, OvAE1171, OvAE532, OvAE1070, OvAE1613, OvAE1711, OvAE615, OvAE398 | > 80 |
| C13 family asparaginyl endopeptidase | legumain (O. viverrini) | OvAE1624, OvAE1824 | 94 |
| S1 family serine protease | HtrA-like (Macaca nr/Schistosoma est) and kallikrein-like (Schistosoma) peptidases | OvAE622, OvAE1918 | 47–53 |
| A1 family aspartic protease | cathepsin D-like; digestive enzyme in helminths (Clonorchis) | OvAE1300 | 80 |
| M41 family metalloprotease | mitochondrial membrane proteinase (Schistosoma) | OvAE65 | 91 |
| Granulin | mitogen associated with cancer (Bos nr/Clonorchis est) | OvAE1732 | 45 |
| Aquaporin | water channel protein (Schistosoma) | OvAE6 | 48 |
| Tyrosinase | critical for S. mansoni egg shell production (Schistosoma) | OvAE1900, OvAE1854 | 63 |
| Phospholipase A2 | similar to vertebrate venom proteins; (Heloderma nr/Clonorchis est) | OvAE1644 | 55 |
| Thioredoxin peroxidase | immunomodulatory in fasciolosis (Schistosoma) | OvAE54 | 74 |
| EF-hand secreted Ca2+-binding protein | calumenin (Rattus nr/Xenopus est) | OvAE61 | 47 |
| Saposin-like protein | pore forming; similar to fluke cytolysins (Clonorchis) | OvAE1692 | 64 |
| Pathogenesis related protein | similar to helminth venom allergen homologues (Schistosoma) | OvAE534, OvAE1862 | 38 |
| Glutathione-S-transferase | detoxification of heme and free radicals (Clonorchis) | OvAE1057, OvAE1892, OvAE1601, OvAE1729 | 86 |
| Synaptobrevin | neurotransmission/vesicular docking – vesicle associated (Schistosoma) | OvAE1001 | 73 |
| Innexin | integral membrane protein forming gap junctions (Schistosoma) | OvAE631 | 78 |
| Fibroblast growth factor (FGF) receptor substrate 2 | host FGF is essential for growth of schistosomes (Schistosoma) | OvAE1563 | 32 |
| Ly6c | Immune cell differentiation antigen | OvAE82 | 26 |