Table 2.

Confirmation of microarray results via real time PCR.

Gene accession	Gene name	Microarray data		Real time PCR data
		Signal intensity (pixel)	relative expression	relative expression
High expression
	actin	33512	1	1
XM_645064	ehcp-a1	17691	0.528	0.853
XM_645550	ehcp-a2	32474	0.969	0.853
XM_645845	ehcp-a5	8628	0.257	0.368
Intermediate
XM_648987	ehasp22-1	2156	0.064	0.020
XM_652272	ehcp-a6	802	0.024	0.026
XM_650130	ehsp 9-2	974	0.029	0.035
XM_651453 XM_650504 XM_647071	ehmp20-3	2838	0.085	0.360
XM_651374	ehmp48-1	895	0.027	0.020
Low
XM_648162	ehcp-a3	549	0.016	0.000
XM_651510	ehcp-a4	414	0.012	0.013
XM_652354	ehcp-a8	510	0.015	0.010
XM_647728	ehasp 22-2	458	0.014	0.000
XM_652471	ehasp 22-3	450	0.013	0.010
XM_648604	ehasp 22-4	516	0.015	0.000
XM_651670	ehsp28-1	380	0.011	0.010
XM_646997	ehsp 28-4	525	0.016	0.020
XM_650302	ehmp8-1	542	0.016	0.015
XM_647540	ehmp8-2	686	0.020	0.010
XM_649181	ehmp49-1	406	0.012	0.023
XM_647901	ehcp-b9	144	0.004	0.000
XM_651336	ehmp20-1	282	0.008	0.000
XM_643899	ehsp 28-2	230	0.007	0.009

Gene expression profile of selected peptidases in E. histolytica isolate HM-1:IMSS.

For microarray analysis nine independent experiments were performed. The signal intensity is indicated in pixel. For real time PCR analysis all primer sets were run in duplicates. Two biological replicates were investigated. The expression of actin as normalizer was set to 1. The relative expression of the peptidases were related to actin.