Figure 3.

NUA Is Located at the Nuclear Envelope during Interphase and in the Vicinity of the Spindle during Prometaphase.

Immunofluorescence images of root tip cell files in interphase ([A] to [D] and [I] to [L]), prometaphase ([A] to [D]), metaphase ([E] to [H]), and late cytokinesis ([I] to [L]). Green, anti-NUA; red in (D), (H), and (L) and magenta in (B), (C), (F), (G), (J), and (K), anti-tubulin; blue in (D), (H), and (L), 4′,6-diamidino-2-phenylindole. (A), (E), and (I) show the green channel only, and (B), (F), and (J) show the red channel only (false colored in magenta). (C), (G), and (K) show the red and green channels, and (D), (H), and (L) show the red, green, and blue channels. The arrowhead in (A) indicates the interphase nuclear envelope, and the arrow in (A) indicates the spindle-like structure in prometaphase. Bars = 10 μm in (D), (H), and (L).