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Poly(A)+ RNA Accumulates in Nuclei of nua Mutants.
Whole-mount in situ hybridization of 10-d-old seedling petioles with fluoresceine-labeled oligo(dT) probe shows increasing severity of nuclear poly(A)+ mRNA retention in nua-2, nua-3, and nua-1. Insets show single nuclei at a higher magnification. All images were taken at identical gain settings. The experiment was repeated twice. Bars = 20 μm.
