Figure 1.

Expression of GFP-PtdInsP 5-Kinase Genes in the Transgenic Tobacco Cells.

(A) RT-PCR analysis. RNA was isolated from wild-type and transgenic tobacco cell lines harvested on day 4 of the culture cycle. Equal amounts of RNA from each sample were reverse transcribed and subjected to RT-PCR using GFP, PIPK, and actin-specific primers. The full-length fusion transcripts were identified with a combination of GFP forward and the selective kinase reverse primers (top panel). Amplification of actin is shown to indicate that equal amounts of RNA were used from each sample. NT-1 (wild type); GFP (GFP vector control); At PIPK10-2, At PIPK10-4, and At PIPK10-5 (three independent transgenic lines transformed with GFP fused to Arabidopsis PIPK10); Hs PIPKIα-1, Hs PIPKIα-2, and Hs PIPKIα-3 (three independent transgenic lines transformed with GFP fused to human PIPKIα).

(B) Growth curve of wild-type and transgenic cell lines over the culture cycle. The fresh weight of two replicate 25-mL cultures was measured on each day. The values are averages (sd) of duplicates from two experiments.