Figure 2.

Agrobacterium Transformation Assays in Nb VIP2–Silenced Plants.

(A) Leaf disk tumorigenesis assay. Leaf disks of the Nb VIP2–silenced plants and TRV:00 (control) plants were inoculated with tumorigenic strain A. tumefaciens A348 and incubated on hormone-free Murashige and Skoog (MS) medium.

(B) Quantification of tumors. The number of tumors produced per leaf disk was counted 3 weeks after inoculation. Data represent the mean of two experiments with a minimum of 150 leaf disks each per treatment with their se values shown as error bars. Asterisk denotes significant difference compared with controls using Fisher's least significant difference test at P = 0.05.

(C) Stable transformation assay. Leaf disks from the silenced and TRV:00 plants were infected with a nontumorigenic strain A. tumefaciens GV2260 harboring the binary vector pCAS1 and incubated on CIM with GF.

(D) Effect of VIP2 gene silencing on cell division. The effect of gene silencing on cell division was evaluated by placing uninoculated leaf disks from the silenced and TRV:00 plants on a nonselective CIM. All the experiments were done with at least five biological replicates and repeated two times, and the results were consistent among the replicates. Photographs shown in (A), (C), and (D) were taken 4 weeks after Agrobacterium inoculation.