Figure 4.

Differential Gene Expression of Nb VIP2 upon Infection with Agrobacterium.

Individual leaves of two separate N. benthamiana plants were syringe (needleless) infiltrated with either an avirulent strain Agrobacterium A136 (lacks Ti plasmid; cannot transfer T-DNA) or a T-DNA transfer-competent strain A. tumefaciens GV2260 carrying pBISN1. Leaf samples from the infiltrated area were collected at different times after inoculation, and total RNA was isolated for real-time quantitative PCR. RNA from the buffer-infiltrated N. benthamiana leaves collected at 12 HAI was used as a calibrator to determine the relative amount of Nb VIP2 transcripts. Samples were pooled together from two independent experiments, and the average of two technical replicates is shown.