Figure 3.

Expression Analysis Predicts Distinct but Also Overlapping Expression Patterns for the VFB Genes.

(A) to (G) Transgenic plants expressing promoter:GUS fusions for each of the four VFB genes were examined in bent cotyledon stage embryos (A), flowers (B), leaves (C), roots (D), and root tips (E). Noteworthy is the strong expression of VFB1 in stomata (F) and of VFB4 in lateral roots (G). Representative staining patterns are shown.

(H) RT-PCR analysis of VFB gene expression in flowers (F), stems (ST), roots (R), leaves (L), and seedlings (SD). 35 PCR cycles were needed to obtain significant gene amplification. ACTIN served as a normalization control of the experiment.