Figure 4.

vfb1, vfb2, and vfb3 Mutants Do Not Have Apparent Phenotypes.

(A) The VFB genes are composed of a single exon (black bar). The T-DNA insertion lines characterized in this study are indicated.

(B) RT-PCR (30 cycles) of VFB gene expression in the vfb1-1, vfb2-1, and vfb3-1 mutants (top three panels) reveals that the full-length transcripts are missing from the respective mutants. The T-DNA insertion upstream of the VFB4 open reading frame in GABI_414F09 does not affect VFB4 expression (fourth panel from the top).

(C) RT-PCR (30 cycles) for VFB gene expression in the vfb1-1 vfb2-1 vfb3-1 triple mutants.

(D) Single and triple vfb mutants (5-week-old plants) do not show apparent phenotypes when grown under standard growth conditions.