Figure 5.

Examination of the Effects of BMV MP on RNA1 and RNA2 Trafficking.

(A) Protein gel blot demonstrating that the MP expressed transiently from a CaMV 35S promoter is comparable to the levels expressed from wild-type RNA3.

(B) Trans-expression MP supports RNA1 trafficking. Locations of the BMV components expressed by agroinfiltration are noted in the schematic of the plants. The shaded leaf 3 denotes agroinfiltration of RNA2 and the MP. The RNA gel blot image contains (+)-strand viral RNAs extracted from the leaves as diagrammed in the schematic.

(C) Trans-expression of MP supports trafficking of RNA2. The shaded leaf 3 denotes the site of agroinfiltration with cultures that express RNA2 and the MP.